(A) Labeling of dendritic spines by anti-spinophilin antibody in mature primary neuronal culture. A dendrite with spines labeled with the F-actin binding mushroom toxin phalloidin can be clearly seen to colocalize spinophilin. Axon terminals labeled by antibody to synaptophysin appose some of these spines (arrows).

(B) Confocal projection micrograph of spinophilin immunoreactivity in human cortex showing labeled puncta from deep layer 3.